Analisis Kadar Interferon Gamma Pada Penderita Tuberkulosis Paru dan Orang Sehat

· Lung Infection

Widjaja JT, Jasaputra DK, Roostati RL. J Respir Indones. 2010;30(2):119-24.

Background : Pulmonary tuberculosis is an acute or chronic respiratory infection that has attract attention of health experts around the world because the prevalence continue to increase. Interferon gamma (IFN-γ) is one of the most important cytokine in the pathogenesis of pulmonary tuberculosis. This study aims to assess the levels of IFN-γ in people with pulmonary tuberculosis and healthy people in the community.
Method and samples : the methodology of this study is observational analytic. Samples of blood plasma derived from venous blood of patients with tuberculosis and healthy people. IFN-γ level is measured by Enzyme Linked analysis techniques Immunosorbent Assay (ELISA). Samples were read by spectrophotometer (ELISA reader). The data obtained were analyzed by unpaired t test (independent t test) with a=0.05.
Result : of this study shows that IFN-γ level in people with pulmonary tuberculosis differ significantly with healthy people (p <0.05).
Conclusion : IFN-γ level in pulmonary tuberculosis patients were lower than healthy people and the difference is statistically significant.

Keywords: IFN-γ, lung tuberculosis, tb

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Martin U, Hasibuan P. J Respir Indones. 2010;30(2);112-8.

Objective : Health care workers (HCWs) in developing countries are at risk for nosocomial tuberculosis (TB). In this study, we are going to determine the prevalence of latency tuberculosis among HCWs in H. Adam Malik hospital in Medan.
Material and Methods : It is a cross sectional study, conducted between July and August 2008. Tuberculin skin test (TST) survey was conducted among 100 HCWs and measured the induration size. Afterward, individuals with positive TST result were examined chest x-ray to determine latency TB.
Result : From 100 participants, 53 (53%) had indurations more than 10 mm and 47 (47%) had indurations less than 10 mm. All of the HCWs with positive TST results had normal chest x-ray. Risk factors associated with a positive TST result were age = 35 years (prevalence ratio, 1,6 [ 95% CI 0,71 to 3,60]), length of professional activity > 5 years (prevalence ratio, 3 [ 95% CI 0,76 to 1,82]), historical BCG vaccination (prevalence ratio, 1,1 [ 95% CI 0,15 to 8,38]), and contact with tuberculosis case (prevalence ratio, 1,1 [ 95% CI 0,51 to 2,51]). None of the risk factors that were assessed had statistically significant (p > 0,05).
Conclusions : The prevalence of latency tuberculosis among HCWs in H. Adam Malik hospital in Medan is 53% that is similar to the prevalence latency tuberculosis in developing countries.
Key Words : Tuberculin Skin Test, Latency Tuberculosis, Health Care Workers, latent tuberculosis, tb laten, tuberkulosis laten, tst, mantoux, tes mantoux, healthcare provider, ucok, martin, hasibuan, pantas