Expression Protein p53 mutation in Non-Small-Cell Lung Carcinoma

· Thoracic Oncology

Hanafi AR, Syahruddin E, Hudoyo A, Hidayat H, Suzanna E. J Respir Indones. 2010;30(3):134-45

Introduction. The tumor suppressor gen p53 mutation encodes a protein that inhibits programmed cell death (apoptosis). The protein is expressed in basal cells in normal human epithelium, but no data are available on the frequency or clinical importance of its expression in carcinoma. We studied p53 mutation protein expression in post surgery tissues of patients with non-small cell lung carcinoma (NSCLC) and evaluated the correlation between protein expressions with prognosis of diseases.
Methods. We have performed a retrospective study using 43 parafin block samples of NSCLC patients who were underwent surgery in Persahabatan Hospital during 1997 to 2008. The p53 mutation protein expression were analyzed by immunohystochemical method using a monoclonal antibody specific for p53 mutation protein. The possibility that p53 mutation expression correlated with survival was investigated with the log-rank test Kaplan Meier.
Results. Patients characteristic we found male 25/43 (58,1%) female 18/43 (41,9%) with mean age 56,19 ± 8,3 y.o and mostly age 40 to 60 y.o 33/43 (76,7%). Number of smoker patiens were 31/43 (72,1%) We also detected p53 mutation protein in 16/43 (37,2%) in NSCLC tissues. Regarding histopatology types were 9/18(50%) in squamous-cell carcinomas and 7/25 (28%) in adenocarcinomas. The corellation between positive p53 mutation protein expressions with pathological staging was significant p 0,004, according to T status T1-T2 62,5% and T3-T4 23,8% have had positive p53 mutation protein. Favorable prognostic significance of p53 mutation in patients with NSCLC stage I – II, patients in the positive p53 mutataion survived longer than those in negative with respective median survival durations 28 and 18 months p 0,019. Adenocarcinomas type with p53 mutation median survival 11 months compared with squamous-cell carcinomas 14 months.
Conclusion. Gene p53 mutation protein found in NSCLC tissues and seems to have role in early stage of lung carcinogenesis. Prognostic significance in stage I – II.

Keywords : p53 mutation protein, apoptosis, immunohystochemical, NSCLC, prognosis

Click for detailed article