Oxidative Stress Study: Malondialdehyde Levels and Superoxide Dismutase Activity in Minimal and Advanced Lesion Pulmonary Tuberculosis

· Lung Infection

Yunita Eka Wati, Nunuk Sri Muktiati, Triwahju Astuti

Background : Oxidative stress plays an important role in pathogenesis of many diseases. Many studies established free radicals involvement in pulmonary TB progression. Currently clinicians use chest X-ray to describe extend of lesion in pulmonary TB and administered first category antituberculosis drug for minimal lesion pulmonary TB. The aim of this study was to assess malondialdehyde (MDA) levels as a marker of oxidative stress, and superoxide dismutase (SOD) activity as an antioxidant in minimal and advanced lesion pulmonary TB.
Methods : Study design was cross sectional. There were 2 groups, minimal and advanced lesion pulmonary TB, each consisting of 10 subjects. Plasma MDA were measured with thiobarbituric acid test. Plasma SOD were measured with nitroblue tetrazolium reduction.
Results : Plasma MDA level in advanced lesion pulmonary TB (12.516±3.739 μg/μL) were higher than in minimal lesion (10.582±2.277 μg/μL), but statistically not significant (p=0.145). Plasma SOD activity in minimal lesion pulmonary TB (41.201±5.217 unit/cc) were higher (significant, p=0.00) than advanced lesion (25.264±11.346 unit/cc).
Conclusion : Plasma oxidative stress in minimal and advanced lesion pulmonary TB has no significant different, therefore first category of antituberculosis drug for minimal lesion was an appropriate choice. There was highly significant decreased of plasma antioxidant (SOD) activity with an increased of plasma lipid peroxidation level (MDA). (J Respir Indo. 2013; 33:163-6)

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