Relationship Between Expression of MAGE-A3 with The Histopathology in NSCLC Patients
DOI:
https://doi.org/10.36497/jri.v40i2.108Keywords:
MAGE-A3, forceps biopsy, NSCLCAbstract
Backgrounds: Most patients with lung cancer was diagnosted in advanced stage (around 57%). Those diagnosed at early stage were only 15%. To increase the cure rate and life expectancy, lung cancer detection should be performed early. Melanoma-associated antigen 3 (MAGE-A3) is a testicular cancer antigen and is widely expressed in various types of tumor tissue. MAGE-A3 is expressed in about 35-40% of NSCLC. Previous research reported the expression of MAGE-A3 in lung cancer was 30-50%. To detect tumor antigens, CT antigens of the MAGE family can be detected through real time polymerase chain reaction (RT-PCR). This study aimed to analyze the relationship between MAGE A3 expression and histopathology type from forceps biopsy specimens in NSCLC patients. Methods: This study was a cross-sectional study conducted on 14 patients with lung cancer in September 2018 to February 2019. Subjects underwent force biopsy with guidance from bronchoscopy in the pulmonary surgery room Dr. Soetomo. The analysis used was the Fisher's exact test. Results: There were six subjects with histopathology type of adenocarcinoma (42.9%) and eight subjects (57.1%) with the histopathology of squamous cell carcinoma. Expression of MAGE-A3 was positive in 5 subjects (35.7%). Conclusions: There was no significant relationship between the expression of MAGE-A3 and the type of histopathology. (J Respir Indo. 2020; 40(2): 113-9)Downloads
References
Murray. Biology of Non-small Lung cancer. In: Mason RJ ed. Murray and Nadel's Textbook Of Respiratory Medicine; 5th edition. Elsevier Saunders, Philadelphia. 2010: 1108-82
Asano H, Toyooka S, Tokumo M, Ichimura K, Aoe K, Ito S, Tsukuda K, Ouchida M, Aoe M, Katayama H, Hiraki A, Sugi K, Kiura K, Date H, Shimizu N. Detection of EGFR gene mutation in lung cancer by mutant-enriched polymerase chain reaction assay. Clin Cancer Res 2006;43:12-43.
INFODATIN. Stop Kanker. Pusat Data dan Informasi Kementerian Kesehatan RI. 2015. Available from www.depkes.go.id › infodatin-kanker
Ettinger DS, Wood DE, Akerley W, et al. Non-Small Cell Lung Cancerversion 3.2015. National Comprehensive Cancer Network Clinical Practice Guideline in Oncology.
Ho MY, Tang SJ, Sun KH, Yang W. Review article immunotherapy for lung cancer. Journal of Biomedicine and Biotechnology 2011;4:1-10
Paez JG, Janne PA, Lee JC, et al. EGFR mutations in lung cancer: correlation with clinical response to gefitinib therapy. Science 2004;304:1497-500.
American Cancer Society. Cancer Facts & Figures 2016. Atlanta: American Cancer Society; 2016. Available from http://cancer.org/research/cancerfactstatistics.
Kreider ME, Hansen-Flaschen J, Ahmad NN, Rossman MD, Kaiser LR, Kucharczuk JC, et al. Complications of Video-Assisted Thoracoscopic Lung Biopsy in Patients with Interstitial Lung Disease. AnnThorac Surg. 2007;83:1140-5.
Shital P, Rujuta A, Sanjay M. Transbronchial needle aspiration cytology (TBNA) in endobronchial lesions: a valuable technique during bronchoscopy in diagnosing lung cancer and it will decrease repeat bronchoscopy. J Cancer Res Clin Oncol (2014) 140:809–815
Joshi M, Deshpande JD. Polymerase chain reaction: methods, principles and application. International Journal of Biomedical Reserch 2010;1:81-97.
Jang SJ, Soria JC, Wang L, Hassan KA, Morice RC, Walsh GL, Hong WK, Mao L. Activation of melanoma antigen tumor antigens occurs early in lung carcinogenesis. American Association for Cancer esearch 2001;61:7959-7963.
Luescher U, Noppen C, Schaefer C, Spagnoli GC, Zuber U. Detection of Tumor-associated Antigen Gene Expression in Peripheral Blood by RT-PCR in Combination with The mRNA Isolation Kit for Blood/bone Marrow. Biochemica. 1997; 4:11-13.
Mancar L, Meek DW. MAGE-A Antigens as Targets in Tumour Therapy. J Lung Can. 2012; 324: 126-132.
Valones MAA, Guimaraes RL, Brandao AC, Eleuterio de Souza PR, Carvalho AAT, Principal and application of polymerase cahin reaction. Brazilian Journal of Microbiology 2009;40:1-11.
Mazzone P, Jain P, Arroliga AC, Matthay RA. Bronchoscopy and needle biopsy techniques for diagnosis and staging of lung cancer. Clinics in chest medicine. 2002;23:137-158.
Weynants P, Lethe B, Brasseur F, et al. Expression of MAGE genes by non-small- cell lung carcinomas. Int J Cancer 1994; 56:826-9.
Vansteenkiste JF, Cho BC, Vanakesa T, Pas TD, Zielinski M, et all. Efficacy of the MAGE-A3 cancer immunotherapeutic as adjuvant therapy in patients with resected MAGE-A3-positive non-small-cell lung cancer (MAGRIT): a randomised, double-blind, placebo-controlled, phase 3 trial. The lancet.com/oncology. 2016:1-14.
Casoni GL, Gurioli C, Chhajed PN, Chilosi M, Zompatori M, et al. The value of transbronchial lung biopsy using jumbo forceps via rigid bronchoscope in diffuse lung disease. Monaldi Arch Chest Dis. 2008;69:59-64.
Se J. Jang, Jean CS, Luo Wang, Khaled A. Hassan, Rodolfo C. Morice, et al. Activation of Melanoma Antigen Tumor Antigens Occurs Early in Lung Carcinogenesis. American Association for Cancer Research. 2001;61: 7959 –7963.
Miranda EI MAGE, Biological Functions and Potential Clinical Applications. J.leuk.res. . 2012; 34: 1121-1122.
Gohji K. Expression of MAGE Genes in testicular Germ Cell Tumours. J Urology. 1999; 53 (4): 843-847
Downloads
Additional Files
Published
Issue
Section
License
- The authors own the copyright of published articles. Nevertheless, Jurnal Respirologi Indonesia has the first-to-publish license for the publication material.
- Jurnal Respirologi Indonesia has the right to archive, change the format and republish published articles by presenting the authors’ names.
- Articles are published electronically for open access and online for educational, research, and archiving purposes. Jurnal Respirologi Indonesia is not responsible for any copyright issues that might emerge from using any article except for the previous three purposes.
