CCL2 and CCR2 Expression in Broncoalveolar Lavage Fluid of Cynomolgus Macaque Model Of Asthma

Sela S Mariya, Fitriya N Dewi, Villiandra Villiandra, Yasmina A Pramastri, Diah Iskandriati, Eric Hayes, Joko Pamungkas, R.P Agus Lelana, Ligaya I Tumbelaka, Dondin Sajuthi

Abstract


Background: Animal models are essential for the development and improvement of novel and effective methods for diagnostic and treatment of human diseases. Cynomolgus monkeys have been used as animal model in asthma studies wherein they exhibited different responses to allergen exposure in the airway; some were low responders while others were high responder. CCL2 is a potent chemotactic factor for monocytes and the gene expression was high in animal model of asthma. The aim of this study was to evaluate CCL2 and CCR2 expression between the low and high responders.
Methods: Realtime PCR technique was used to evaluate CCL2 and CCR2 gene expression in bronchoalveolar lavage samples. The subject
of this study was asthmatic cynomolgus monkeys, consisted of 4 low responders and 4 high responders. This study was held in Biotechnology
Laboratory of Primate Research Center LPPM IPB on October 2016- January 2017.
Results: CCL2 and CCR2 expression in low responders were higher than those in high responders at 24hours post airway challenge.
Conclusion: CCL2 may potentially be developed as target for therapy or a genetic marker for asthma responsiveness in individuals. (J Respir Indo 2018; 38(2): 115-22)

Keywords


animal model, asthma, CCL2, cynomolgus monkeys, gene expression

Full Text:

PDF

References


Messaoudi I, Estep R, Robinson B, Wong SW. Nonhuman primate models of human immunology. Antioxid Redox Signal. 2011;14:261–73.

Hessel EM, Chu M, Lizcano JO, Chang B, Herman N, Kell S a, et al. Immunostimulatory oligonucleotides block allergic airway inflammation by inhibiting Th2 cell activation and IgE-mediated cytokine induction. J Exp Med. 2005;202:1563–73.

Shin YS, Takeda K, Gelfand EW. Understanding asthma using animal models. Allergy, Asthma Immunol Res. 2009;1:10–8.

Lambrecht BN, Hammad H. The immunology of asthma. Nat Immunol. 2014;16:45–56.

Hyde DM, Miller LA, Schelegle ES, Fanucchi M V., Van Winkle LS, Tyler NK, et al. Asthma: A comparison of animal models using stereological methods. Eur Respir Rev. 2006;15:122–35.

Badan Penelitian dan Pengembangan Kesehatan. Riset Kesehatan Dasar (RISKESDAS) 2013. Laporan Nasional 2013. 2013.

Afandi S, Yunus F, Andarini S, Kekalih A. Tingkat kontrol pasien asma di Rumah Sakit Persahabatan berdasarkan asthma control test beserta hubungannya dengan tingkat morbiditas dan faktor risiko. Resirology Indones. 2013;33:230–43.

Zhao L, Yang W, Yang X, Lin Y, Lv J, Dou X, et al. Chemerin suppresses murine allergic asthma by inhibiting CCL2 production and subsequent airway recruitment of inflammatory dendritic cells. Allergy Eur J Allergy Clin Immunol. 2014;69:763–74.

Kusano KF, Nakamura K, Kusano H, Nishii N, Banba K, Ikeda T, et al. Significance of the level of monocyte chemoattractant protein-1 in human atherosclerosis. Circ J. 2004;68:671–6.

Rull A, Camps J, Alonso-Villaverde C, Joven J. Insulin resistance, inflammation, and obesity: Role of monocyte chemoattractant protein-1 (orCCL2) in the regulation of metabolism. Mediators Inflamm. 2010;2010:1-11.

Giuffrida MJ, Valero N, Mosquera J, Alvarez de Mon M, Chacín B, Espina LM, et al. Increased cytokine/chemokines in serum from asthmatic and non-asthmatic patients with viral respiratory infection. Influenza Other Respi Viruses. 2014;8:116–22.

Viliandra, Dewi FN, Paramastri Y, Hayes E, Iskandriati D. Sensitivity to Ascaris suum (AS) Extract in Indonesian Macaca fascicularis : Skin Test Reactivity and Airway Challenge. In: P BP, Setijanto H, Agungpriyono S, Purwantara B, Pamungkas J, Djuwita I, et al., editors. Joint Meeting Of The 3rd International Meeting On Asian Zoo/Wildlife Medicine And Conservation (AZWMC 2008) & 10th National Veterinary Scientific Conference Of Indonesian Veterinary Medical Association (KIVNAS X PDHI 2008). Bogor: IPB Press; 2008. p. 143–4.

Brightling CE, Gupta S, Gonem S, Siddiqui S. Lung damage and airway remodelling in severe asthma. Clin Exp Allergy. 2012;42:638–49.

Berair R, Saunders R, Brightling CE. Origins of increased airway smooth muscle mass in asthma. BMC Med. 2013;11:2–7.

Singh SR, Sutcliffe A, Kaur D, Gupta S, Desai D, Saunders R, et al. CCL2 release by airway smooth muscle is increased in asthma and promotes fibrocyte migration. Allergy Eur J Allergy Clin Immunol. 2014;69:1189–97.

Wang C, Huang C, Lin H, Lee K, Lin S, Liu C, et al. Increased Circulating Fibrocytes in Asthma with Chronic Airflow Obstruction. Am J Respir Crit Care Med. 2008;178:583–91.

Schmidt K, Martinez-Gamboa L, Meier S, Witt C, Meisel C, Hanitsch LG, et al. Bronchoalveoloar lavage fluid cytokines and chemokines as markers and predictors for the outcome of interstitial lung disease in systemic sclerosis patients. Arthritis Res Ther. 2009;11:R111.

Palchevskiy V, Hashemi N, Weigt SS, Xue YY, Derhovanessian A, Keane MP, et al. Immune response CC chemokines CCL2 and CCL5 are associated with pulmonary sarcoidosis. Fibrogenesis Tissue Repair. 2011;4:10.

Lin F-C, Chen Y-C, Chang S-C. Clinical importance of bronchoalveolar lavage fluid and blood cytokines, surfactant protein D, and Kerbs von Lungren 6 antigen in idiopathic pulmonary alveolar proteinosis. Mayo Clin Proc. 2008;83:1344–9.

ROSE CE, SUNG S-SJ, FU SM. Significant Involvement of CCL2 (MCP-1) in Inflammatory Disorders of the Lung. Microcirculation. 2010;10:273–88.

Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative C T method. 2008;3:1101–8.

Turabelidze ABS, Guo S, DiPietro LA. Importance of Housekeeping gene selection for accurate RT-qPCR in a wound healing model. Wound Repair Regen. 2011;18:460–6.

Zou J, Young S, Zhu F, Gheyas F, Skeans S, Wan Y, et al. Microarray profile of differentially expressed genes in a monkey model of allergic asthma. Genome Biol. 2002;3:research0020.

Baay-Guzman GJ, Bebenek IG, Zeidler M, Hernandez-Pando R, Vega MI, Garcia-Zepeda E a, et al. HIF-1 expression is associated with CCL2 chemokine expression in airway inflammatory cells: implications in allergic airway inflammation. Respir Res. 2012;13:60.

Yang EJ, Choi E, Ko J, Kim DH, Lee JS, Kim IS. Differential effect of CCL2 on constitutive neutrophil apoptosis between normal and asthmatic subjects. J Cell Physiol. 2012;227:2567–77.

Mellado M, de Ana AM, Gomez L, Martinez-A C, Rodriguez-Frade JM. Chemokine Receptor 2 Blockade Prevents Asthma in a Cynomolgus Monkey Model. J Pharmacol Exp Ther. 2007;324:769–75.

Hansbro PM, Kaiko GE, Foster PS. Cytokine/anti-cytokine therapy - Novel treatments for asthma? Br J Pharmacol. 2011;163:81–95.

Chelbi H, Ghadiri A, Lacheb J, Ghandil P, Hamzaoui K, Hamzaoui A, et al. A polymorphism in the CCL2 chemokine gene is associated with asthma risk: a case-control and a family study in Tunisia. Genes Immun. 2008;9:575–81.

Cho YB, Lee WY, Choi S-J, Kim J, Hong HK, Kim S-H, et al. CC chemokine ligand 7 expression in liver metastasis of colorectal cancer. Oncol Rep. 2012;28:689–94.

Tian H, Bharadwaj S, Liu Y, Ma PX, Atala A, Zhang Y. Differentiation of human bone marrow mesenchymal stem cells into bladder cells: potential for urological tissue engineering. Tissue Eng Part A. 2010;16:1769–79.




DOI: https://doi.org/10.36497/jri.v38i2.166

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2018 Jurnal Respirologi Indonesia

INDEXING & PARTNER

SINTA Garuda Indonesian Scientific Journal Database (ISJD) Indonesia One Search (IOS) Crossref

ROAD-ISSN Dimensions Google Scholar 

 
Jurnal Respirologi Indonesia
pISSN: 0853-7704 - eISSN: 2620-3162
Address: Jalan Cipinang Bunder No. 19, Cipinang, Pulogadung, Jakarta Timur, DKI Jakarta 13240, Indonesia
Phone: +62-21-2247-4845
Email: editor@jurnalrespirologi.org

An official publication by
the Indonesian Society of Respirology (ISR)

Creative Commons License
Creative Commons Attribution-ShareAlike 4.0 International License

Statcounter