Email the author The Comparison of TGF-β Levels in Post Pulmonary Tuberculosis Patients with and Without Smoking History in Haji Adam Malik General Hospital Medan
Abstract
Methods: This was an analytic study with cross sectional design, conducted from October 2016 to February 2017 in RSUP H. Adam Malik Medan. All subjects had recovered from TB, confirmed by clinical, radiological and bacteriological examination. Smoking history of the subjects was assessed using Brinkman Index. The TGF-β measurement was performed using venous blood sample processed through Enzyme Link Immunosorbent Assay (ELISA) by means of TGF-β kit.
Results: This study included post-pulmonary TB patients, of which 26 subjects were smokers and 25 were non-smokers, consisted of 31 males and 20 females. The mean TGF-β level of all samples was 6690.5±4913.4 mg/ml. The mean TGF-β level in smokers was 6621.5±4856.7 mg/ml, while in non-smokers 6762.2±5071 mg/ml. Statistical analysis using Mann-Whitney test revealed that there were no significant differences of TGF-β level in smokers and non-smokers among post-pulmonary TB patients (P=0.618).
Conclusions: There were no significant differences of TGF-β level in smokers and non-smokers among post-pulmonary TB patients. (J Respir Indo. 2020; 40(3): 139-43)
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Ramos LMM, Sulmonett N, Ferreira CS, Henriques JF, de Miranda SS. Functional profile of patients with tuberculosis sequelae in a university hospital. J Bras Pneumol [Internet]. [cited 2019 Apr 26];32(1):43–7. Available from: http://www.ncbi.nlm.nih.gov/pubmed/17273568
Tarigan AP, Pandia P, Eyanoer P, Tina D, Pratama R, Fresia A, et al. Obstructive lung disease as a complication in post pulmonary TB. IOP Conf Ser Earth Environ Sci [Internet]. IOP Publishing; 2018 Mar [cited 2019 Apr 26];125(1):012154. Available from: http://stacks.iop.org/1755-1315/125/i=1/a=012154?key=crossref.47e30c74f3a99be1404aff198f4881a3
Laurent GJ, McAnulty RJ, Hill M, Chambers R. Escape from the matrix: multiple mechanisms for fibroblast activation in pulmonary fibrosis. Proc Am Thorac Soc [Internet]. American Thoracic Society; 2008 Apr 15 [cited 2019 Apr 26];5(3):311–5. Available from: http://www.ncbi.nlm.nih.gov/pubmed/18403325
Tatler AL, Jenkins G. TGF-β Activation and Lung Fibrosis. Proc Am Thorac Soc [Internet]. 2012 Jul 15 [cited 2019 Apr 26];9(3):130–6. Available from: http://www.atsjournals.org/doi/abs/10.1513/pats.201201-003AW
Uhal BD, Joshi I, Hughes WF, Ramos C, Pardo A, Selman M. Alveolar epithelial cell death adjacent to underlying myofibroblasts in advanced fibrotic human lung. Am J Physiol Cell Mol Physiol [Internet]. 1998 Dec [cited 2019 Apr 26];275(6):L1192–9. Available from: http://www.ncbi.nlm.nih.gov/pubmed/9843857
Pardo A, Selman M. Molecular mechanisms of pulmonary fibrosis. Front Biosci [Internet]. 2002 Aug 1 [cited 2019 Apr 26];7:d1743-61. Available from: http://www.ncbi.nlm.nih.gov/pubmed/12133818
Checa M, Hagood JS, Velazquez-Cruz R, Ruiz V, García-De-Alba C, Rangel-Escareño C, et al. Cigarette Smoke Enhances the Expression of Profibrotic Molecules in Alveolar Epithelial Cells. PLoS One [Internet]. Public Library of Science; 2016 [cited 2019 Apr 26];11(3):e0150383. Available from: http://www.ncbi.nlm.nih.gov/pubmed/26934369
DiFazio RM, Mattila JT, Klein EC, Cirrincione LR, Howard M, Wong EA, et al. Active transforming growth factor-β is associated with phenotypic changes in granulomas after drug treatment in pulmonary tuberculosis. Fibrogenesis Tissue Repair [Internet]. BioMed Central; 2016 [cited 2019 Apr 26];9:6. Available from: http://www.ncbi.nlm.nih.gov/pubmed/27148404
Mattila JT, Ojo OO, Kepka-Lenhart D, Marino S, Kim JH, Eum SY, et al. Microenvironments in tuberculous granulomas are delineated by distinct populations of macrophage subsets and expression of nitric oxide synthase and arginase isoforms. J Immunol [Internet]. NIH Public Access; 2013 Jul 15 [cited 2019 Apr 26];191(2):773–84. Available from: http://www.ncbi.nlm.nih.gov/pubmed/23749634
Ariyothai N, Podhipak A, Akarasewi P, Tornee S, Smithtikarn S, Thongprathum P. Cigarette smoking and its relation to pulmonary tuberculosis in adults. Southeast Asian J Trop Med Public Health [Internet]. 2004 Mar [cited 2019 Apr 26];35(1):219–27. Available from: http://www.ncbi.nlm.nih.gov/pubmed/15272772
Mak JCW, Leung HCM, Sham ASK, Mok TYW, Poon YN, Ling SO, et al. Genetic polymorphisms and plasma levels of transforming growth factor-β1 in Chinese patients with tuberculosis in Hong Kong. Cytokine [Internet]. Academic Press; 2007 Dec 1 [cited 2019 Apr 26];40(3):177–82. Available from: https://www.sciencedirect.com/science/article/abs/pii/S1043466607004152
Olobo JO, Geletu M, Demissie A, Eguale T, Hiwot K, Aderaye G, et al. Circulating TNF-alpha, TGF-beta, and IL-10 in Tuberculosis Patients and Healthy Contacts. Scand J Immunol [Internet]. John Wiley & Sons, Ltd (10.1111); 2001 Jan 1 [cited 2019 Apr 26];53(1):85–91. Available from: http://doi.wiley.com/10.1046/j.1365-3083.2001.00844.x
Godinas L, Corhay J-L, Henket M, Guiot J, Louis R, Moermans C. Increased production of TGF-β1 from sputum cells of COPD: Relationship with airway obstruction. Cytokine [Internet]. 2017 Nov [cited 2019 Apr 26];99:1–8. Available from: http://www.ncbi.nlm.nih.gov/pubmed/28668545
Zuiker RGJA, Kamerling IMC, Morelli N, Calderon C, Boot JD, de Kam M, et al. Reproducibility of biomarkers in induced sputum and in serum from chronic smokers. Pulm Pharmacol Ther [Internet]. 2015 Aug [cited 2019 Apr 26];33:81–6. Available from: http://www.ncbi.nlm.nih.gov/pubmed/25966831
Magnan A, Frachon I, Rain B, Peuchmaur M, Monti G, Lenot B, et al. Transforming growth factor beta in normal human lung: preferential location in bronchial epithelial cells. Thorax [Internet]. BMJ Publishing Group; 1994 Aug [cited 2019 Apr 26];49(8):789–92. Available from: http://www.ncbi.nlm.nih.gov/pubmed/8091325
Tzortzaki EG, Tsoumakidou M, Makris D, Siafakas NM. Laboratory markers for COPD in "susceptible" smokers. Clin Chim Acta [Internet]. 2006 Feb [cited 2019 Apr 26];364(1–2):124–38. Available from: http://www.ncbi.nlm.nih.gov/pubmed/16139829
DOI: https://doi.org/10.36497/jri.v40i3.110
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