Clinical and Demographic Characteristics Differences between M. tuberculosis and M. bovis Infection in Bronchoalveolar Lavage Pulmonary TB Patients, Indonesia

Budi Yanti, Soetjipto Soetjipto, Ni Made Mertaniasih, Muhammad Amin

Abstract


Background: Some species of the Mycobacterium tuberculosis complex (MTBC) which can cause tuberculosis, particularly M. tuberculosis and M. bovis, may have different virulence property and therefore associated with clinical severity in tuberculosis (TB) patients. The aim of this study was to determine the differences in clinical and demographic characteristics between M. tuberculosis and M. bovis infection among Indonesian TB patients.
Methods: Thirty one new and active TB patients were confirmed to have AFB sputum and gene Xpert for M. tuberculosis from Dr. Soewandhie Hospital, Surabaya, Indonesia. Interviews were conducted to record the clinical and demographics required. MTBC were isolated from bronchoalveolar lavage fluid (BALF) and determined by primer-specific PCR targeting TbD1 and RD9 region gene. The degree of lung tissue damage was classified using NICE Scoring System.
Results: MTBC were detected in all patients on whom 19 and 12 isolates were classified as M. tuberculosis and M. bovis respectively. Age of infection was significantly different between M. tuberculosis and M. bovis (p<0.005). There was no difference in clinical characteristics of tuberculosis. The degree of lung damage was significantly different between M. tuberculosis and M. bovis (OR: 17.00; 95% CI: 2.55-112.98), p=0.002).
Conclusions: M. tuberculosis is likely different in ages and increases the risk of severe lung damage seventeen times among TB patients from Indonesia. Distinguishing the clinical characteristics of these two species infection still difficult. So, it is necessary for laboratory tests that integrated with the National Tuberculosis Control Program in this country to determine between this species.

Keywords


M. tuberculosis, M. bovis, Bronchoalveolar lavage, clinical, demographic

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DOI: https://doi.org/10.36497/jri.v39i4.76

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Jurnal Respirologi Indonesia
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