Email the author Effect of Moringa Leaf Extract in Inhibiting Lung Fibrosis in Wistar Rats with Diabetic Model
Abstract
Background: Uncontrolled diabetes can lead to macrovascular and microvascular complications in various organs. Organ fibrosis is one of the microvascular complications due to diabetes. Hyperglycemia triggers ROS formation thereby causing oxidative stress. Oxidative stress enhances pro-inflammatory and pro-fibrotic activities. The lungs have a lot of vascularization and connective tissue, making them susceptible to diabetes complications. Moringa is called 'The Miracle Tree' because it has many properties. Moringa leaves are the most studied part, for their efficacy as antioxidants. This study examines the effect of moringa leaf extract (MLE) in inhibiting lung fibrosis in diabetic Wistar rats.
Methods: A total of 27 male Wistar rats were randomized into three groups, K0, K1 and P. Group K0 was given saline (ip) and saline (orally); K1 was given Streptozotocin (STZ) (ip) and saline (orally); P was given STZ (ip) and MLE (orally). MLE was administered daily at 1000 mg/Kg BW dose for four weeks. Lung fibrosis assessment was carried out using a modified Ashcroft scale.
Results: Lung fibrosis assessment showed that the K1 group had the highest mean of lung fibrosis and K0 had the lowest mean of lung fibrosis. Kruskal-Wallis test showed that there was a significant difference in the data comparison between the three groups. The post-hoc test showed that there was a significant difference in the data comparison between groups. Comparison of the K0-K1 group data showed the smallest significance value and comparison of the K0-P group data showed the highest significance value.
Conclusion: MLE can inhibit lung fibrosis in diabetic Wistar rats.
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International Diabetes Federation. IDF diabetes atlas 10th edition. 10th ed. Magliano D, Boyko E, editors. Vol. 102, International Diabetes Federation. 2021.
Kemenkes RI. Pola Hidup Sehat dan Deteksi Dini Bantu Kontrol Gula Darah Pada Penderita Diabetes. Serina IV. Jakarta; 2021.
Chawla A, Chawla R, Jaggi S. Microvasular and macrovascular complications in diabetes mellitus: Distinct or continuum? Indian Journal of Endocrinology and Metabolism. 2016;20:546–51.
Tuleta I, Frangogiannis NG. Diabetic fibrosis. Biochimica et Biophysica Acta - Molecular Basis of Disease. 2021;1867:166044.
Shah MS, Brownlee M. Molecular and cellular mechanisms of cardiovascular disorders in diabetes. Circulation Research. 2016;118:1808–29.
Mameli C, Ghezzi M, Mari A, Cammi G, Macedoni M, Redaelli FC, et al. The diabetic lung: Insights into pulmonary changes in children and adolescents with type 1 diabetes. Metabolites. 2021;11(2):69.
Ehrlich SF, Quesenberry CP, Van Den Eeden SK, Shan J, Ferrara A. Patients diagnosed with diabetes are at increased risk for asthma, chronic obstructive pulmonary disease, pulmonary fibrosis, and pneumonia but not lung cancer. Diabetes Care. 2010;33(1):55–60.
Essa MM, Subash S, Parvathy S, Meera A, Guillemin GJ, Memon MA, et al. Brain health benefits of Moringa oleifera. In: M. M. Essa MAMMA, editor. Food and Brain Health. New York: Nova Science Publishers; 2014. p. 113–8.
Aminah S, Tezar R, Yanis M. Kandungan nutrisi dan sifat fungsional tanaman kelor (Moringa oleifera). Buletin Pertanian Perkotaan Balai Pengkajian Teknologi Pertanian Jakarta. 2015;5(2):35–44.
Shih MC, Chang CM, Kang SM, Tsai ML. Effect of different parts (leaf, stem and stalk) and seasons (summer and winter) on the chemical compositions and antioxidant activity of Moringa oleifera. Int J Mol Sci. 2011;12(9):6077–88.
Hernández-Rodríguez P, Baquero LP, Larrota HR. Flavonoids: Potential therapeutic agents by their antioxidant capacity. In: Bioactive Compounds: Health Benefits and Potential Applications. Woodhead Publishing; 2019. p. 265–88.
Gianosa G, Suryono S, Santosa A. Renoprotective activity of Morinaga oleifera lamk toward kidney injury rats induced by streptozotocin. NurseLine Journal. 2022;7(1):21–7.
Kasolo JN, Bimenya GS, Ojok L, Ogwal-Okeng JW. Sub-acute toxicity evaluation of Moringa oleifera leaves aqueous and ethanol extracts in Swiss Albino rats. International Journal of Medicinal Plant Research. 2012;1(6):75–81.
Hübner RH, Gitter W, El Mokhtari NE, Mathiak M, Both M, Bolte H, et al. Standardized quantification of pulmonary fibrosis in histological samples. Biotechniques. 2008;44(4):507–11.
Schuyler MR, Niewoehner DE, Inkley SR, Kohn R. Abnormal lung elasticity in juvenile diabetes mellitus. American Review of Respiratory Disease. 1976;113(1):37–41.
Davis TME, Knuiman M, Kendall P, Vu H, Davis WA. Reduced pulmonary function and its associations in type 2 diabetes: The Fremantle Diabetes Study. Diabetes Res Clin Pract. 2000;50(2):153–9.
Hu Y, Ma Z, Guo Z, Zhao F, Wang Y, Cai L, et al. Type 1 diabetes mellitus is an independent risk factor for pulmonary fibrosis. Cell Biochemistry and Biophysics. 2014;70:1385–91.
Susanto H, Yunisa DT, Taufiq A, Putra WE, Jannah NR, Putri SA, et al. Anti fibrogenesis effect of green materials Moringa oleifera leaf powder (MOLP) on the progression of hepatocellular carcinoma. In: AIP Conference Proceedings. 2021. p. 2353.
Wilujeng LK, Safitri FN, Supriono S, Kalim H, Poeranto S. The effect of Moringa oleifera (Lam) leaves ethanol extracts as anti-inflammatory and anti-fibrotic through TNF-α and p38-MAPK expression: In vivo model of liver fibrosis approach. In: AIP Conference Proceedings. 2021. p. 2353.
He B sai, Wang X, Zhang Y, Gao C, Wu C kai, Guo S rui, et al. Anti-oxidant, anti-inflammatory, and anti-fibrotic effects of Moringa oleifera seeds on renal injury diabetic induced by streptozotocin. J Funct Foods. 2022;95(4):105168.
Awodele O, Oreagba IA, Odoma S, Teixeira Da Silva JA, Osunkalu VO. Toxicological evaluation of the aqueous leaf extract of Moringa oleifera Lam. (Moringaceae). J Ethnopharmacol. 2012;139(2):330–6.
Saleem A, Saleem M, Akhtar MF, Baig MMFA, Rasul A. HPLC analysis, cytotoxicity, and safety study of Moringa oleifera Lam. (wild type) leaf extract. J Food Biochem. 2020;44(10):e13400.
Alia F, Putri M, Anggraeni N, Syamsunarno MRAA. The potency of Moringa oleifera Lam. as protective agent in cardiac damage and vascular dysfunction. Frontiers in Pharmacology. 2022;12:724439.
Margiana R, Liem IK, Pawitan JA. Microscopic study of the lungs of sprague-dawley rats from various ages of postnatal and aging periods. Online J Biol Sci. 2015;15(2):74–82.
Calabresi C, Arosio B, Galimberti L, Scanziani E, Bergottini R, Annoni G, et al. Natural aging, expression of fibrosis-related genes and collagen deposition in rat lung. Exp Gerontol. 2007;42(10):1003–11.
Thrall RS, McCormick JR, Jack RM, McReynolds RA, Ward PA. Bleomycin-induced pulmonary fibrosis in the rat. Inhibition by indomethacin. American Journal of Pathology. 1979;95(1):117–30.
Gomes GF, Pisani JC, Macedo ED, Campos AC. The nasogastric feeding tube as a risk factor for aspiration and aspiration pneumonia. Current Opinion in Clinical Nutrition and Metabolic Care. 2003;6:327–33.
Blumenstein I, Shastri YM, Stein J. Gastroenteric tube feeding: Techniques, problems and solutions. World Journal of Gastroenterology. 2014;20:8505–24.
Samareh Fekri M, Poursalehi HR, Najafipour H, Dabiri S, Shokoohi M, Siahposht Khacheki A, et al. Pulmonary complications of gastric fluid and bile salts aspiration, an experimental study in rat. Iran J Basic Med Sci. 2013;16(6):790–6.
Chiang CC, Chen CM, Suen JL, Su HH, Hsieh CC, Cheng CM. Stimulatory effect of gastroesophageal reflux disease (GERD) on pulmonary fibroblast differentiation. Digestive and Liver Disease. 2020;52(9):988–94.
DOI: https://doi.org/10.36497/jri.v44i3.534
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